Aflatoxin M1 Determination in Ice Cream Based on Immunoaffinity Column Clean-up Followed by HPLC-FLD

Background: Aflatoxin M1 (AFM1) in industrial ice cream was analyzed by immunoaffinity column (IAC) clean-up followed high-performance liquid chromatography (HPLC) with a fluorescence detector (FLD) as highly-sensitive method to confirm and quantify AFM1 the samples. Methods: A total of 150 samples were randomly collected from supermarkets seven Iranian cities (Tehran, Tabriz, Karaj, Urmia, Sanandaj, Qazvin, Khoramabad). Results: detected more than 80.0% The average concentration 29.79 ng/L. According results, 12.62% samples, levels exceeded maximum European Union (EU) limits for adults (50 ng/mL). This study recommends regular monitoring Iran. Conclusion: Results this indicate need develop database assist law enforcement agencies, traders, policymakers local governments minimize or prevent health-related risks caused AFM1.